= Make Stack Image =
File – Import – Image Sequence ….

= Rotation =
Plugins – eigene – Rotation

= Stack align =
Macro – Edit – ImageJ/plugins/eigene/stackalign.txt
On stackalign window – Macros – Run Macro
Crop

= Reg affine =
Plugins – StackReg – Affine
Crop

= Max Int =
Plugins – eigene – polfft punktuell max 2four me

= Needle Direction =
Plugins -eigene- auto polfft ratio-15-autothres short.txt
-> Thresdhold : Median
-> 10
-> 5

Overlay Setting : defualts
Autopolfft : defualts

Orientation-1 -> needle direction infomation