= Make Stack Image =
File – Import – Image Sequence ….
= Rotation =
Plugins – eigene – Rotation
= Stack align =
Macro – Edit – ImageJ/plugins/eigene/stackalign.txt
On stackalign window – Macros – Run Macro
Crop
= Reg affine =
Plugins – StackReg – Affine
Crop
= Max Int =
Plugins – eigene – polfft punktuell max 2four me
= Needle Direction =
Plugins -eigene- auto polfft ratio-15-autothres short.txt
-> Thresdhold : Median
-> 10
-> 5
Overlay Setting : defualts
Autopolfft : defualts
Orientation-1 -> needle direction infomation